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Joshee, Nirmal

Department: 
Ag Research
Location: 
116 Stallworth
Phone: 
478-825-6373; Lab 478-822-1290
Email: 
josheen@fvsu.edu
Fax: 
478-825-6376

Nov. 1, 1998- June 30, 2001. University of Nebraska Med Ctr, Omaha, NE 68131, Liposome mediated gene transfer in animal cells (Post Doctoral)
Sept. 1991-March 1995. Biotechnology Institute, Ogata, Akita. Japan. Molecular biology of water stress induced genes (Post Doctoral)
Teaching:
Graduate teaching (MS Biotechnology)

Faculty Bio
Education: 

Over 20 years of Experience
M.S. Botany, Ph.D.

Publications: 

Tascan, A, J. W. Adelberg, A. M. Rimando, M. Tascan, N. Joshee, A. K. Yadav. 2010. Hyperhydricity and Flavonoid Content of Scutellaria Species In Vitro on Polyester-supported Liquid Culture Systems. HortScience (Accepted)
•Tascan, A, J. W. Adelberg, M. Tascan, N. Joshee, A. K. Yadav. 2009. Polyester fiber controlled hyperhydricity for three species of Scutellaria: Medicinal plant. Acta Hort. (ISHS) 826:141-146.
•Wei Hao, Rajeev Arora, N. Joshee, and A. K. Yadav. 2009. Freezing tolerance and cold acclimation in guava (Psidium guajava L.). HortScience 44: 1258-1266.
•Joshee, N. 2009. Paulownia: A Multipurpose Tree for Rapid Lignocellulosic Biomass Production. In: Handbook of Bioenergy Crops (Eds. C. Kole, C. P. Joshi, and D. Shonnard). Taylor and Francis.
•Joshee, N., Rimando, A. M., Parajuli, P., Rawat, G. S., and Yadav, A. K. 2009. Investigating two medicinal Scutellaria species of Himalayan origin. In: Advances in Agriculture, Environment, and Health; Fruits, Vegetables, Animals and Biomedical Sciences (Eds. Singh SB, Chaurasia, OP, Yadav, A, Rimando, AM, and Terrill, TH). Pp. 347-356. SS Publishing House, Delhi, India.
•P. Parajuli, N. Joshee, A. Rimando, S. Mittal and A. K. Yadav. 2009. In vitro anti-tumor mechanisms of various Scutellaria extracts and constituent flavonoids. Planta Medica 75:41-48.
•Tascan A., Jeff Adelberg, Nirmal Joshee, A K Yadav, and M, Tascan. 2007. Liquid culture systems for Scutellaria spp. Acta Hort. 756:163-170.
•Joshee, N, D. Harris, A, Yadav, and A. K. Yadav. 2007. Influence of explant selection and culture conditions on organogenesis and germplasm conservation in Bacopa monnieri (L) Wettst. Acta Hort. 756: 119-128.
•Joshee, N, S R Mentreddy, and A K Yadav. 2007. Mycorrhizal fungi and growth and development of micropropagated Scutellaria integrifolia plants. Industrial Crops and Production 25(2):169-177.
•Yadav, A., Joshee, N., and A. K. Yadav. 2007. Regeneration of multiple shoots from immature seeds and mass multiplication of dhatura (Datura metel L.). Acta Hort. 756:199-210.
• Joshee, N., B. K. Biswas, and A. K. Yadav. 2007. Somatic embryogenesis and plant development in Centella asiatica L., A highly prized medicinal plant of tropics. HortScience 42(3):633-637.
•Biswas, B K, Joshee, N, Yadav, A, Yadav, A K. 2007. Development and application of biotechnology in Guava: a nutraceutical fruit. Acta Hort 744: 267 - 276.
•Joshee, N, Mutua, M, Zee, F., and Yadav, A. K. 2004. In vitro shoot bud induction and plantlet regeneration in guava as influenced by genotype. Acta Hort (ISHS) 632:279-285.
•Joshee N, Bastola D, and Cheng P-W. 2002. Transferrin-facilitated lipofection gene delivery strategy: characterization of the transfection complexes and intracellular trafficking. Hum. Gene Therapy 13:1991-2004.
•Joshee, N, T. S. Patrick, Rao, S. Mentreddy, and A. K. Yadav. 2002. Skullcap: Potential medicinal crop. In: Jules Janick (editor), Perspectives on New Crops and New Uses, American Society Hort. Sci. Press, Alexandria, VA, pp. 581-587.
•Bastola, D R, Agrawal, V P, and Joshee N., 2000. In vitro propagation of a Himalayan pine- P. wallichiana A. B. Jacks. Curr. Sci. 78(3):338-341.
•Sinha, S, Pokhrel, S, Vaidya, B, Joshee, N, 1999. Rapid in vitro micropropagation and callus induction in Scutellaria discolor Colebr.- A medicinally important plant of Nepal. Indian J. Pl. Genetic Resources 12 (2/3):219 -223.
•Li, C, Joshee, N, Adler,K B and Cheng, P-W, 1999. Development of monoclonal antibodies against bovine mucin core-2β-6 N-acetylglucosaminyltransferase. Glycoconjugate Journal 16: 555-562.
•Joshee N, Kisaka H, and Kitagawa Y, 1998. Isolation and characterization of a water- stress specific genomic gene, pwsi18, from rice. Plant Cell Physiol. 39 (1): 64 -72.
•Takahashi R., Joshee N. and Kitagawa Y, 1994. Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress regulated genes in rice. Plant Mol. Biol. 26: 339-352.

Research Interests: 

Medicinal Plants and Lignocellulosic Ethanol
Medical Plants: tissue culture and conservation of medicinal plants; reporductive bilogy, plant tissue and protoplast culture, anther and microspore culture, Development of transgenic plants with desirable tratis; Hairy root culture and Phytochenical screening for bioactive flavonoids; Evaluation of bioactive molecules for apoptosis/necrosis using prostate, breast, and glioma cells; Plant germplasm conservation and artificial seed prodcution
*Micropropagation Research:
Forest trees-pine, Ficus, Artocarpus; Orchids-symbiotic seed germination (Dendrobium, Dactylorhiza, Vanda); Medicinal-Scutellaria, Solanums, Centalla, Bacopa; Ornamentals-african violet, carnation, chrysanthemum; Plantation and Cereal crops-Banana and rice (tissue and anther culture)
*Molecular Biology Research: Regulation of stress induced genes in plants; Isolation, sequencing, and characterization of stress induced genes inplants; Liposome mediated gene transfer in cancer cell lines for gene therapy

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