Mahipal Singh, Ph.D.
- College: College of Agriculture, Family Sciences and Technology
- Department: Agricultural Sciences Academic Department
- Title: Associate Professor
- Associated Majors and Graduate Programs: Animal Science
- Associated Majors and Graduate Programs: Biotechnology (Master of Science)
Department: Agricultural Sciences
Official Job Title: Associate Professor of Animal Biotechnology, and Coordinator of Animal Science Program
Campus Address: Stallworth Biotech Building #117
Ph.D., Zoology-Microbiology, Banaras Hindu University, Varanasi, India (1989)
M.S., Zoology, Institute of Advanced Studies, Meerut University Meerut, India (1983)
B.S. (Hons), Zoology, Meerut College Meerut, Meerut University, India (1978)
- 2017-(April 15-July 15)-Visiting Scientist, USDA-ARS Beltsville Agricultural Research Center (BARC), MD
- 2013-todate; Associate Professor of Animal Biotechnology, Fort Valley State University
- 2007-2013; Assistant Professor of Animal Biotechnology, Fort Valley State University
- 2004-2007; Research Assistant Professor, University of Illinois, & Jesse Brown VA Medical Center, Chicago, IL
- 2000-2003; Adjunct Biology/MPH Graduate Faculty & Research Associate, Fort Valley State University
- 1995-2000; Scientist (1995-97); Senior Scientist (1997-2000), Division of Biotechnology, CSIR Institute of Himalayan Bioresource Technology, Palampur, India
- 1993-1995; Pool Officer, Center for Molecular Biology, National Botanical Research Institute, Lucknow, India
- 1991-1993; International Visiting Fellow, National Institute of Child Health and Human Development, NIH, Bethesda, MD
- 1989-1991; Postdoctoral Fellow, Infectious Diseases, Medical University of South Carolina, Charleston, SC
- ANSC-6303-Molecular Biology Techniques
- ANSC-6253-Molecular Endocrinology
- ANSC-6421-Graduate Seminars
- ANSC-2813-Animal Biotechnology
- ANSC-4841-Animal Science Seminar
- BIOT-5434-Molecular Genetics and Bioinformatics (team taught)
- USDA-ARS 1890 Faculty Research Fellowship Award (2017)
- STEM Research Excellence Award, Fort Valley State University (2014-15)
- First Prize for undergraduate student Oral Research Presentation, FVSU Annual Research Day symposium (2017)
- Second Prize for Graduate student Oral Research Presentation, FVSU Annual Research Day symposium (2017)
- Second Prize for student Research Poster, FVSU Annual Research Day symposium (2016)
- Best Poster Award, National Symposium on Biotechnology, CIMAP, Lucknow, India (1999)
- Fogarty International Visiting Fellowship, National Institutes of Health, Bethesda, MD (1991-93)
- Senior Research Fellowship, Council of Scientific & Industrial Research, Govt. of India (1987-89)
- Annual Seminar Competition winner, Research Fellows’ Forum, Central Drug Research Institute, Lucknow, India (1986)
- Junior Research Fellowship, Council of Scientific & Industrial Research, Govt. of India (1984-86)
- Junior Research Fellowship, University Grants Commission, Govt. of India (1983-84)
- All India Science Essay Contest Winner (4th Prize), Competition Success Review Magazine (1978)
- Merit Scholarship, Govt. of Utter Pradesh, India (1976-78)
- Student of the Year Award (for all round performance), DC Inter College Mohana, Bulandshahar, India (1975)
STUDENT PROJECTS SUPERVISED/THESIS COMMITTEES CHAIRED
(Total 38 students):
4 PhD: Chandan Sharma (1996-2001), Bandana Dhiman (1997-2001), Dharam Singh (1998-2001), Jyoti Raizada (1998-2001), all at IHBT Palampur, India; 13 MS: Jyoti Saroop (1998), Jaspreet Singh (1999), Amit Rathi (1999), Rajeev K. Kaushal (2000), all at IHBT Palampur; Charles Okonkwo (2011-2013), Venkata Degala (2012-2014), Hadj S. Aoued (2013-2015), Olabisi T. Ojo (2013-2015), Wesley Spratling (2014-2016), Brian Walcott (2014- 2016), Charnice McDonald (2015-17), Benjamin Hortman (2015-17), Tyler Williams (Sp2017- current), all at FVSU; 7 BS: Karisna Muniz (2003), Tarneisha Williams (Spring 2009), Oberziner White (2010-11), Jared Tolbert (Spring 2011), Mobley Melissa (Fall 2011), Colby Hicks (2013), Christine Hicks (Fall 2013), Kya McKinje (Fall 2016); 8 HS: Tamara Brown (2001), Shy Richardson (2008), Oberziner White (2009), Arpita Yadav (2009), Colby Hicks (2010), Whitney N. Little (2012), Akash Arora (Fall 2015 & summer 16), Sahan Vangala (Summer 2016) and; 6 Middle School students: Aamanya Raval (Fall 2009), Namrata Buxani (Fall 2010), Navin Buxani (Fall 2010), Shelly Vyas (Fall 2010), George Lu (Fall 2011), Ishan Vyas (Fall 2016).
Editorial Board [Journal of Biotech Research (2008-todate]; Grant Reviewer [Ad-hoc Reviewer USDA-NIFA-SBIR & USDA-NIFA-BRAG programs (2010-11)]; USDA-NIFA-Evans-Allen program (since 2009); Panelist, K-14 partnerships and recruitment/retention of students, USDA/NIFA DOCE Project Directors Meeting, Virginia Tech, June 25, 2013; Collaborative panel II, EMPIRE Conference of FVSU, The King & Prince Beach & Golf Resort, St. Simons Island, GA, Dec. 12-14, 2016; Journal Reviewer, reviewing for about 10 Journals on a regular basis; Coordinator, Monthly Research Seminars, College of Agriculture, FVSU (2008-14); Committees, [Undergraduate Research Program Advisory Council (2016-17); Institutional Promotion and Tenure Committee (2016-17); Graduate faculty member (since 2008); Provost & Vice President for Academic Affairs search committee (2016); Faculty Senator (representing Agricultural Research) (2012-14; 2016-17); Graduate Council (2015-17); Honors Convocation Committee (2013 & 2014); Co-chair, special awards subcommittee (in 2013); Local Organizing Committee of the Society for In Vitro Biology Annual Meeting (2014); Agricultural & Lab animal Care and Use Committee (ALACUC) (2013-todate); Ad hoc member, Animal Science and Biotechnology Graduate Admissions Committees (since 2008); Animal Science PhD Program Exploratory Committee (2010-11)]; Society Member [American Society of Animal Science, Society for the Study of Reproduction, Indian Science Congress, Society for In Vitro Biology]. Moderator/Judge [ARD Symposiums, K12 Science Fairs of Houston County Schools (since 2008); FVSU Annual Research Day Symposium (2016)]; CSIR (India) Sports Promotion Board (1999-2000); CSIR-IHBT Liaison Officer (1997-98); Bio-Safety & Recombinant DNA Committees of IHBT Palampur, India (1997-2000).
Broader areas: Animal biotechnology; molecular biology; in-vitro cell culture; genome-editing; stem cells; livestock reproduction.
Area 1: Recovery of live and proliferative cells from postmortem tissues: This project was initiated to understand the postmortem cellular life. How long the individual cells in tissues remain alive after the death of an animal? Could we recover and utilize the stem proliferative cells from such bygone tissues and use them for cellular therapies and preservation of germplasm for future generations? These are some of the questions being studied using cellular and molecular approaches. We have recently discovered that individual cells remain alive in mammalian tissues for much longer time than was previously thought. Please see our publications and some of the images assembled in the figure below in this write up.
Area 2: genome-editing in livestock: This project aims at reducing milk allergens and mastitis in goats using CRISPR/Cas technology in collaboration with Dr. David Donovan of USDA-ARS, Beltsville, Dr. Telugu Bhanu of University of Maryland at College park, and Dr. George McCommon of FVSU.
Area 3: Myostatin gene-targeting: This project aims at understanding the role of myostatin, a member of the TGF-beta family of proteins, in muscle development. This research area has gained considerable interest in recent years due to its potential in the treatment of muscular dystrophy in humans and in enhancing meat production in agriculture to meet the future demand of protein to feed the expanding world population. Our efforts are targeted towards enhancing muscular mass in meat goats using modern molecular, cellular, and reproductive tools combined with traditional breeding approaches to extend the desired traits.
Earlier research: Epigenetic regulation of globin gene expression during mammalian development & cellular differentiation; sickle cell disease, rRNA genes; DNA vectors. Antimicrobial drug-resistance, diarrheal diseases, DNA damage, and repair mechanisms, Chloroplast engineering for high expression of bacterial genes, DNA fingerprinting, DNA based detection of adulteration in herbal medicine, environmental pollution.
(out of 130)
Peer-reviewed Journal Articles:
- Brian W.; and Singh. Recovery of proliferative cells up to 15- and 49-days postmortem from bovine skin stored at 25°C and 4°C, respectively. Cogent Biology (2017) 3: 1333760 (https://doi.org/10.1080/23312025.2017.1333760).
- Singh, M; and N Degala. Postmortem recovery of proliferative fibroblasts up to three days in livestock ear skin stored at 35°C. International Journal of Biology. (2017) 9(2): 9-13. (https://doi.org/10.5539/ijb.v9n2p9)
- Singh, M; K. McKenzie, and X. Ma. Effect of dimethyl sulfoxide on in vitro proliferation of skin fibroblast cells. Journal of Biotech Research (2017) 8: 78-82.
- Aoued, HS; and Singh. Recovery of Fibroblast-Like Cells after 160 Days of Postmortem Storage of Goat Skin Tissues in Refrigerated Media. Journal of Veterinary Science and Technology (2015) 6: 4 (DOI: 10.4172/2157-7579.1000236)
- Okonkwo, C; and Singh. Recovery of fibroblast-like cells from refrigerated goat skin up to 41 days of animal death. In Vitro Cell and Developmental Biology-Animal. (2015) 51: 463-69 (DOI: 10.1007/s11626-014-9856-9)
- Singh, M; and X. Ma. In Vitro Culture of Fibroblast-Like Cells From Sheep Ear Skin Stored at 25-26°C for 10 Days After Animal Death. International J. of Biology (2014) 6 (4): 96-102 (doi:10.5539/ijb.v6n4p96)
- Mehta R; R Arya, K Goyal, M Singh and A K Sharma. Bio-preservative and therapeutic potential of pediocin: recent trends and future perspectives. Recent Patents on Biotechnology (2013) 7(3): 172-78 (doi: 10.2174/18722083113076660008)
- Adefenwa, MA, B O Agaviezor, SO Peters, M Wheto, K Adekoya, M Okpeku, B Oboh, GO Williams, M Singh and IG Imumorin. Identification of ingle nucleotide polymorphisms in the agouti signaling protein (ASIP) gene in Nigerian and North American goats. Molecular Biology Reports (2013) 40(7): 4447-4457 (doi: 10.1007/s11033-013-2535-1)
- Singh, M; X. Ma & A. Sharma. Effect of postmortem time interval on in vitro culture potential of goat skin tissues stored at room temperature. In Vitro Cell and Developmental Biology-Animal. (2012) 48: 478-482 (doi: 10.1007/s11626-012-9539-3)
- Singh, M; Dhiman, C. Sharma. Characterization of a highly repetitive DNA sequence in Camellia sinensis (L.) O. Kuntze genome. Journal of Biotech Research (2011) 3: 78-83
- Singh, M; X Ma, E Amoah and G Kannan. In vitro culture of fibroblast-like cells from postmortem skin of Katahdin sheep stored at 4 degree C for different time intervals. In Vitro Cell and Developmental Biology-Animal. (2011) 47(4):290-93. (doi: 10.1007/s11626-011-9395-6).
- Singh, M; AK Sharma & P Yadav. Characterization of GSF289: a fibroblast cell line derived from goat ear skin explants. Journal of Biotech Research. (2011) 3: 1-6.
- Singh, M & A Sharma. Outgrowth of fibroblast cells from goat skin explants in three different culture media and the establishment of cell lines. In Vitro Cell and Developmental Biology-Animal. (2011) 47 (10): 83-88. (doi: 10.1007/s11626-010-9373-4).
- Lavelle, D; Y Saunthararajah; K Vaitkus; M Singh; V Banzon; P Phiasivongsva; S Redkar; S Kanekal; D Bearss; C Shi; R Inloes & J DeSimone. S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons ( Anubis). Journal of Translational Medicine (2010) 8:92 (doi:10.1186/1479-5876-8-92)
- Singh, M; AS Yadav; X Ma & E Amoah. Plasmid DNA transformation in Escherichia Coli: effect of heat shock temperature, duration, and cold incubation of CaCl2 treated cells. International Journal of Biotechnology & Biochemistry (2010) 6(4):561–568.
- $Chin, J; $M Singh; V Banzon; M Hankewych; T Kousnetzova; V Ibanez; J DeSimone & D Lavelle. Transcriptional activation of the gamma-globin gene in baboons treated with decitabine and in cultured erythroid progenitor cells involves different mechanisms. Experimental Hematology (2009) 37(10):1151-1142 ($ equal contribution by Drs. Chin and Singh) (doi:10.1016/j.exphem.2009.06.007)
- Singh, M & B Dhiman. Construction and characterization of a gene-targeting plasmid DNA vector in Nicotiana tabacum. Journal of Biotech Research (2009) 1: 13-20.
- Lavelle, D; J Chin; K Vaitkus; S Redkar; T Chunlin; M Hankewych; M Singh; Y Saunthararajah & J DeSimone. Oral decitabine mesylate reactivates expression of the methylated γ-globin gene in Papio anubis. American Journal of Hematology. (2007) 82(11): 981-985. (http://dx.doi.org/10.1002/ajh.21020)
- Singh, M; K Vaitkus; M Hankewych; D Lavelle; N Mahmud & J DeSimone. The γ-globin gene promoter progressively demethylates as the hematopoietic stem progenitor cells differentiate along the erythroid-lineage in baboon fetal liver and adult bone marrow. Experimental Hematology (2007) 35: 48-55 (http://dx.doi.org/10.1016/j.exphem.2006.09.001)
- Lavelle, D; K Vaitkus; M Hankewych; M Singh & J DeSimone. Developmental changes in DNA methylation and covalent histone modifications of chromatin associated with the ε-, γ- and β-globin gene promoters in Papio anubis. Blood Cells, Molecules & Diseases (2006) 36(2): 269-278.
- Lavelle, D; K Vaitkus; M Hankewych; M Singh & J DeSimone. Effect of 5-aza-2’-deoxycytidine (Decogen) on covalent histone modifications of chromatin associated with the ε-, γ- and β-globin promoters in Papio anubis. Experimental Hematology (2006) 34: 339-347.
- Singh, M; J Saroop & B Dhiman. Detection of intra-clonal genetic variability in vegetatively propagated tea using RAPD markers. Biologia Plantarum (2004) 48: 113-115.
- Singh, M; C Sharma; B Dhiman; D Singh & J Raizada. Cloning and characterization of repetitive DNA sequence elements from Camellia sinensis (L) O. Kuntze. Physiology and Molecular Biology of Plants (2004) 10(2): 209-215.
- Mondal, TK; HP Singh, M Lakshmikumaran, M Singh, AK Gupta & PS Ahuja. RAPD markers specific to China tea (Camellia sinensis). Proceedings of the International Conference on O-CHA (tea) culture and science (2004): pp 137-140.
- *Dhiman, B & M Singh. Molecular detection of Cashew husk (Anacardium occidentale) adulteration in market samples of dry tea (Camellia sinensis). Planta Medica (2003) 69: 882-884. (*Article highlighted on the cover page of the journal).
- Singh, D & M Singh. Organization of 5S ribosomal RNA genes in tea (Camellia sinensis). Genome (2001) 44(1): 143-146. (http://dx.doi.org/10.1139/gen-44-1-143)
- Singh, M; C Sharma & PS Ahuja. A heterologous chloroplastic rDNA revealed highly conserved RFLP patterns in the family Asteraceae. Plant Molecular Biology Reports (1999) 17(1): 73. (http://dx.doi.org/10.1023/a:1007510818291)
- Singh, M. Application of RAPD fingerprinting in plant micropropagation industry. Every Man’s Science (1999) 34(2): 76-79.
- Singh, M; B Dhiman & PS Ahuja. Isolation and PCR amplification of genomic DNA from market samples of dry tea. Plant Molecular Biology Reporter (1999) 17(2): 171-178. (http://dx.doi.org/10.1023/a:1007562802361)
- Singh, M & C Sharma. Detection of plasmid-encoded gusA gene in a GUS positive Escherichia coli. BioTechniques (1999) 26(2): 261-264.
- Singh, M. Mammalian cloning: implications on our society. Indian Veterinary Medical Journal. (1998) 22(2): 133-135.
- Singh, M. Hematological responses of a fresh water teleost Channa punctatus to experimental copper and chromium poisoning. Journal of Environmental Biology. (1995) 16(4): 339-341.
- Woodgate, R; M Singh; OI Kulaeva; EG Frank; AS Levine & WH Koch. Isolation and characterization of novel plasmid-encoded umuC mutants. Journal of Bacteriology. (1994) 176(16): 5011-5021.
- Singh, M; SC Sanyal; MA Choudhary & JNS Yadav. Plasmid-mediated drug-resistance in veterinary and human isolates of Escherichia coli: A potential threat to human and veterinary medicine. Indian Veterinary Medical Journal. (1994) 18(3): 131-141.
- Singh, M & JNS Yadav. Evidence of a 35.8 x 108 Dalton Ent-R-plasmid in Escherichia coli isolated from human urinary-tract infection. Indian Veterinary Medical Journal. (1992) 16(1): 51-53.
- Singh, M; JNS Yadav & SC Sanyal. Enterotoxigenic drug-resistant plasmids in animal isolates of Escherichia coli and their zoonotic importance. Journal of Tropical Medicine and Hygiene. (1992) 95(5): 316-321.
- Singh, M & JNS Yadav. Are the oxytetracycline and doxycycline resistance genes different? Indian Veterinary Medical Journal. (1992) 16(1): 82-86.
- Singh, M; MA Choudhry; JNS Yadav & SC Sanyal. The spectrum of antibiotic-resistance in human and veterinary isolates of Escherichia coli collected from 1984-86 in Northern India. Journal of Antimicrobial Chemotherapy. (1992) 29: 159-168. (http://dx.doi.org/10.1093/jac/29.2.159)
- Singh, M & JF John. Isomeric DNA ladder formation of a plasmid encoding tobramycin resistance in Escherichia coli. Journal of Antimicrobial Chemotherapy. (1991) 28(5): 627-637. (http://dx.doi.org/10.1093/jac/28.5.627)
- Singh, M; MA Choudhry & JNS Yadav. Drug-resistance and colicin production among various serotypes of Escherichia coli of animal origin. Indian Journal of Pathology and Microbiology. (1989) 32(3): 161-166.
- Choudhry, MA; M Singh & JNS Yadav. Critical concentration of lincomycin and polymixin-B in Biken Assay for detection of heat-labile enterotoxins of Escherichia coli. Letters in Applied Microbiology. (1988) 7: 123-125. (http://dx.doi.org/10.1111/j.1472-765x.1988.tb01307.x)
- Singh, M & JNS Yadav. Effect of acridinium ions on curing of R-plasmids. Indian Journal of Experimental Biology. (1988) 26(10): 668-670.
- Ahmad, M; JNS Yadav & M Singh. Enterotoxigenicity and antibiotic-resistance behavior of Escherichia coli of animal origin. Indian Journal of Medical Research. (1988) 83(3): 466-472.
- Yadav, JNS; M Ahmad & M Singh. Prevalence of enterotoxigenic Escherichia coli in the clinical isolates of man and animals. Indian Journal of Animal Sciences. (1988) 58(5): 532-34.
- Singh, M & JNS Yadav. Prion: the smallest pathogen (a review). Indian Veterinary Medical Journal. (1985) 9(4): 216-222.
- Yadav, JNS & M Singh. Genetic stability of various R-factors among clinical isolates of Escherichia coli under normal laboratory conditions. Indian Veterinary Medical Journal. (1985) 9(3): 123-131.
- Singh, M & VP Agarwal. Lanasyn dark-violet induced heamatochemical anomalies in a teleost Heteropneustes fossilis. Journal of Agricultural and Biological Research. (1985) 1(1): 16-19.
- Singh, M; AK Awasthi & VP Agarwal. Lanasyn dark-violet induced enzymological probes in the liver and kidney of a fresh water teleost Heteropneustes fossilis. Indian Veterinary Medical Journal. (1985) 9(3): 163-165.
- Singh, M; C Sharma & B Lal. Species-specific DNA sequences and their utilization for identification of Viola species and in detection of adulteration in a medicinal herb “banafsha”. US patent no: 6465637 granted in 2001.
- Singh, M & B Dhiman. Species-specific genomic DNA sequences for identification of Anacardium occidentale and the method for their utilization in detection of adulteration of cashew husk in tea samples. US patent no: 6541624 granted in 2001.
- Ahmad, I; MSA Khan, F Aqil & M Singh*. Microbial applications in agriculture and the environment: a broad perspective. In: Iqbal Ahmad, F. Ahmad & J. Pichtel. Microbes and Microbial Technology: Agriculture and Environmental Applications; Springer New York (2011) pp.1-27. (doi: 10.1007/978-1-4419-7931-5_1) (*Corresponding author).
- Ahmad, I; MSA Khan, FM Husain, M Zahin & M Singh. Bacterial Quorum Sensing and Its Interference: Methods and Significance. In: Iqbal Ahmad, A. Ahmad and J. Pichtel. Microbes and Microbial Technology: Agricultural and Environmental Applications; Springer New York (2011) pp.127-161. (doi: 10.1007/978-1-4419-7931-5_6)
- Tuli, R; PVAL Ratnekar; J Kapila; A Srivastava; N Vijayalakshmi; HD Rananavare; HS Mishra; SK Gupta; M Singh & PK Singh. Development of transgenic plants for resistance to insect attack. In: K K Tewari & G S Singhal. Eds. Plant Molecular Biology and Biotechnology, Narosa Publishing House, New Delhi (1997) pp 37-42.
Popular science articles:
- Singh, Solar ultra-violet radiation and life on earth: from primitive to modern. Himachal Reporter (2000) 1(12): Page 6, Column 1-3 and continued in later issue i.e. 1(13): Page 4, Column 1-2.
- Singh, M. DNA technology and hope for innocent: Indian scenario. Palampur Reporter (1998) 6(L): Page 4, column 1-3.
- Singh, M. DNA Fingerprinting: Revolutionary applications of a chance discovery. Palampur Reporter (1996) 4(16): Page 2, Column 3-5.
- Singh, M. Eke suksham roganu: Prion (in Hindi). Congress Vernica (1988) 5(2):52.
- Singh, M. Badh Ko rokne ke kuchh sujhav (in Hindi). Vigyan Pragati (1982) 10(353): 436-438.
- $Singh, M. Science in the service of rural development. Competition Success Review (May, 1979)
$This article won fourth prize in All India Essay Contest No. 287 conducted by Competition Success Review, a premier monthly periodical of India
DNA sequences published in public database GENBANK:
- Adefenwa,M.A., Agaviezor,B.O., Peters,S.O., Wheto,M., Adekoya,K.O., Okpeku,M., Oboh,B., Williams,G.O., Singh, M., De Donato,M., Thomas,B. and Imumorin,I.G. Capra hircus clone 94 melanocortin 1 receptor (MC1R) gene (& association with coat color), partial cds. GenBank Accession Number:
- Singh, M; E Amoah; G Kannan, SL Stice & DM Donovan. Capra hircus myostatin upstream region genomic sequence. Accession number:
- Singh, D & M Singh. Structure of 5S ribosomal RNA gene repeat units in Camellia sinensis. Accession number: AY101390
- Singh, M; B Dhiman, C Sharma; D Singh (2002): Cloning, sequencing and characterization of a highly repetitive DNA fragment from Camellia sinensis (O.) Kuntze genome. [Include (AG)n microsatellites]. Accession number: AF546881
- Singh, M & AK Yadav. Organization of 5S ribosomal RNA gene in Carica quercifolia. Accession number: AF506758
- Singh, M; C Sharma & B Lal. Viola species-specific DNA sequences. Accession numbers: AR237797, AR237798, AR237799, AR237800, AR237801, AR237802, AR237803, AR237804, AR237805, AR237806, AR237807 and AR237808 (parts of Singh patent)
- Singh, M & B 5S ribosomal RNA intergenic spacer regions of detective value isolated from Anacardium occidentale, Camellia sinensis, and Dendrocalamus hamiltonii. Accession numbers: AY230649, AY230650 and AY230651, respectively.
Abstracts published (in conferences/society meeting proceedings):
- #,*McKinzie, K; X. Ma and Singh. Effect of Dimethyl Sulfoxide (DMSO) on In Vitro Proliferation of Mammalian Cells. Presented by my undergraduate student in 18th Biennial ARD Research symposium, Atlanta, GA April 2-5, 2017.
- #,* Hortman, M. Singh, X. Ma, and N. Degala Establishment of Caprine Mammary Luminal Epithelial Cell Lines. FVSU 7th Annual Research Day, April 20, 2017. Abstract No: 23, pp 19. (Third Prize winner).
- #,*McKinzie, K; and Singh. Effect of Dimethyl Sulfoxide (DMSO) on goat skin fibroblast cells in In Vitro culture assays. Presented by my undergraduate student in FVSU 7th Annual Research Day, April 20, 2017. Abstract No: 6, pp 13. (First Prize winner)
- * Singh and Brian Walcott. In vitro culture of cells up to 15- and 49-days postmortem in bovine skin stored at 25°C and 4°C, respectively. Presented in American Society of Cell Biology meeting, San Francisco, CA, December 3-7, 2016; Poster no: P879 (Board no: B252)
- * Singh and X. Ma. Recovery of fibroblast cells upto 65 days of postmortem storage of sheep ear skin at 4°C. Presented in American Society of Animal Science meeting, Salt Lake City, July 17-19, 2016.
- $,#Spratling, W; S. A. Aoued, O. J. Ojo, A.I. Younis, E. Amoah and Singh. Comparison of oocyte recovery from slaughtered animal ovaries in three livestock species and their quality assessment. FVSU 6th Annual Research Day, April 14, 2016. Abstract No: 16, pp 14-15. (2nd Prize winner)
- Singh* and Brian Walcott. Isolation of Fibroblast-like Cells after 42 Days of Postmortem Storage of Cattle Skin in a Refrigerator. In Vitro Cell. Dev. Biol.-Animal (2016) 52 (Suppl.1): page 6 (Abstr.). Poster no: A-3000. Presented in the World Forum on In Vitro Biology, Davis CA, June 11-15, 2016.
- #,*Aoued, H.S., Singh. Recovery of fibroblast-like cells after 160 days of postmortem storage of goat skin tissues in refrigerated media. In Vitro Cell. Dev. Biol.-Animal (2014) 50: S40. DOI 10.1007/s11626-014-9765-y (Abstr.). Interactive poster presentation no: A2002.
- *Singh, M; X. Ma, G. Kannan, and E. Amoah. Time limits of postmortem cell survival in goat ear skin stored at room temperature. Animal Sci. (2013) 91 (E. Suppl.): pp220 (Abstr).
- Singh, X. Ma, C. Okonkwo, N. Degala, P. Yadav, O. White, E. Amoah, W. Getz, and C. Borne. Enhancing student learning with hands-on laboratory exercises and research projects in animal biotechnology. NACTA Journal, Vol. 57, supplement 1 (2013) pp 96-97, abstract no 132. Presented in NACTA Conference, at Virginia Institute of Technology, June 25-29, 2013.
- Singh, M; and X. Ma. Post-mortem Recovery of Fibroblast-like Cells in Sheep after 10 Days of Animal Death. In Vitro Cell. Dev. Biol.-Animal (2013) 49: S40-46. DOI: 10.1007/S11626-013-9605-5. (Abstr.). Interactive poster presentation no: A2000.
- *Singh, M; and X. Ma. Sheep Skin Fibroblast Cell Cultures from Room Temperature Stored Tissues after Ten Days of Animal Death. 17th Biennial ARD Research symposium, Gainesville, FL April 7-10, 2013, Abstract No: 206. Page 120.
- #,*Okonkwo, C; X. Ma and Singh. Postmortem Cell Survival Pattern of Goat Skin Tissues Stored in Refrigerator at 4°C. 17th Biennial ARD Research symposium, Gainesville, FL April 7-10, 2013, Abstract No: 11, page 50.
- #,*White, O; X. Ma, and Singh. Evaluation of Fetal Goat Serum as a Cheaper Alternative to Fetal Bovine Serum for Culturing Goat Cells. 17th Biennial ARD Research symposium, Gainesville, FL April 7-10, 2013, Abstract No: 31, Page 57.
- #Degala, V.N.; X. Ma, E. Amoah, and Singh. Development of a Cell Biological Tool for Genetic Modification of Goats. 17th Biennial ARD Research symposium, Gainesville, FL April 7-10, 2013, Abstract No: 319, page 162.
- Singh, M; X. Ma and A. K. Sharma. In vitro culture of fibroblast-like cells from room temperature stored goat tissue. In Vitro Cell. Dev. Biol.-Animal (2012) 48: S50-51. (Abstr.).
- *Singh, M; and X. Ma. Ability to culture of cells from postmortem goat skin tissues stored at room temperature for different time intervals. Animal Sci. (2012) 90 (Suppl.3): 674 (Abstr).
- *Singh, Can dead animals be revived after several days of their death? Oral presentation given in 2nd FVSU Research Day, April 12, 2012. Abstract No: O7, p34
- Singh, Can superior trait animal genetics be preserved after several days of animal death? Presented as a poster in FVSU Research Day, April 19, 2011. Abstract No: 26
- Singh, M; Sharma. Isolation, culture and characterization of a fibroblast cell line from skin samples of Saanen breed of goats. In Vitro Cell. Dev. Biol.-Animal (2011) 47 (Suppl 1): S41-42. (Abstr.) DOI: 10.1007/S11626-011-9409-4. Interactive poster presentation
- Singh, M & A. Sharma. Establishment of Fibroblast Cell Lines from Goat Ear Skin Explants. Presented in 16th Biennial ARD Research symposium, Atlanta, GA April 9-13, 2011, Abstract No; O-FS-S-15 page 63
- Singh, M; X. Ma, S. Richardson, E. Amoah and G. Kannan. Recovery of Live Fibroblast-like Cells in Sheep at Different Times Postmortem. Presented in 16th Biennial ARD Research symposium, Atlanta, GA April 9-13, 2011; Abstract No: P-FS-S-11, PAGE 82-83
- #,*White, O; X Ma & M Singh. Screening of 10-Nucleotide Long Random Primers for Goat Genotyping Using Polymerase Chain Reaction. Presented in 16th Biennial ARD Research symposium, Atlanta, GA April 9-13, 2011, Abstract no: O-FS-U-10, page 51
- Singh, M; A Sharma; X Ma; E Amoah & G Kannan. Construction of a goat myostatin gene promoter-GFP translational fusion to study promoter regulation. Journal of Animal Science. (2010) 88 (E-Suppl.3): 8. (Abstr.)
- #,*White, O; M Singh & DL Daniels. Screening of small 10-nucleotide long randam primers for goat genotyping. Presented in the 5th annual symposium and research conference of Peach State-Louis Stokes Alliance for minority participation (LSAMP) at UGA, Sept. 24-25, 2010. Abs on page 37.
- Singh, M; E Amoah; G Kannan; SL Stice & DM Donovan. Genome walk of an unknown upstream region of myostatin gene in Spanish goats. Presented in the “Frontiers of Reproductive Biology and Regulation of Fertility” meeting of KeyStone Symposia on Molecular and Cellular Biology, Hilton Hotel, SantaFe, NM. February1-6, 2009. Abs. No. 219, pp65
- Singh, M; E Amoah; G Kannan & T Terrill. Cloning and Partial Characterization of the Upstream Promoter Region of the Myostatin Gene in Spanish Goats. Presented in 15th Biennial ARD symposium, Hyatt Regency Atlanta, GA, March 28-April 1, 2009. Abs. No 182, pp.107.
- #,*Richardson, S; EA Amoah; J Gosier; M Jones & Singh. Oocyte recovery from slaughtered goats during non-breeding season. Presented in 15th Biennial ARD symposium, Hyatt Regency Atlanta, GA March 28-April 1, 2009. Abs. No 135, pp.90.
- Lavelle, D; J Chin; K Vaitkus; M Singh; M Hankewych & J DeSimone. The pattern of covalent histone modifications throughout the β-globin gene locus in fetal liver and adult bone marrow erythroblasts pre- and post-decitabine. Blood Cells, Molecules & Diseases (2007) 38(2):
- Lavelle, D; J Chin; K Vaitkus; S Redkar; P Phiasivongsa; T Chunlin; R Will; M Hankewych; M Singh; Y Saunthararajah & J DeSimone. Oral decitabine reactivates fetal hemoglobin in baboon (Papio anubis). Blood Cells, Molecules & Diseases (2007) 38(2):
- Singh, M; K Vaitkus; M Hankewych; D Lavelle & J DeSimone. Decitabine targets the erythroid progenitor/precursor subpopulations for the γ-globin gene demethylation in baboon. Blood (2006) 108: 556.
- Lavelle, D; K Vaitkus; M Singh; M Hankewych & J DeSimone. Recapitulation of the fetal pattern of expression of the Iγ and Vγ-globin genes in cultured baboon CD34+ bone marrow cells. Blood (2006) 108: 1590.
- Lavelle, D; M Singh; K Vaitkus; M Hankewych & J DeSimone. Polymorphisms within the baboon (P anubis) γ-globin gene promoter reduce DNA methylation density and may determine HbF levels. Blood Cells, Molecules and Diseases (2005) 34(2):101-102.
- Singh, M; K Vaitkus; M Hankewych; D Lavelle; N Mahmud & J DeSimone. Epigenetic changes in the γ-globin gene promoter in hematopoietic progenitor cells at different stages of differentiation. Blood (2005) 106(11): 495a.
- Lavelle, D; K Vaitkus; M Hankewych; M Singh & J DeSimone. Changes in globin gene methylation and covalent histone modifications of chromatin associated with the ε-, γ- and β-globin promoters of the anubis during development. Blood (2004) 104 (11): 341a.
- Lavelle, D; K Vaitkus; M Hankewych; M Singh & J DeSimone. Effect of decitabine on covalent histone modifications of chromatin associated with the ε-, γ- and β-globin gene promoters in baboon. Blood (2004) 104 (11): 111a.
- Lavelle, D; M Singh; K Vaitkus; M Hankewych & J DeSimone. Polymorphic Pvu II sites in the stage selector elements of the baboon γ-globin gene promoter may determine HbF levels by reducing DNA methylation density. Blood (2004) 104(11): 343a.
- Singh, M; K Knowles & S Dhir. Expression of GFP reporter gene in Arundo donax following microprojectile bombardment. Society for In-Vitro Biology, Annual Meeting, Portland, Oregon, May 31-June 4, 2003. Abs No: P-2018.
- Singh, M; D Moore, K Knowles & S Dhar. In-vitro plant regeneration of Arundo donax through somatic embryogenesis. Society for In-Vitro Biology, Annual Meeting, May 31-June 4, 2003. Portland, Oregon, Abs No: P-2062.
- Singh, M & AK Yadav. Cloning and partial characterization of 5S ribosomal RNA gene repeat units from two Carica species using PCR. 10th International Association for Plant Tissue Culture & Biotechnology congress, Orlando, Florida, June 23-28, 2002. Abs. No: P-1274.
- Gosukonda, RM; M Singh & AK Yadav. Optimal levels of antibiotics to eliminate Agrobacterium and in-vitro selection in guava. HortScience (2001) 36(3): 535
- Sharma, C; M Singh; B Lal; PS Ahuja & AK Gupta. Germplasm characterization in Artemisia species collected from Himachal Pradesh using RAPD markers. Society of Biological Chemists of India, 68th Annual Meeting, Indian Institute of Science, Bangalore, India, December 26-28, 1999. Abs. No: P-14.
- #,$Madanala, R; SK Gupta; M Singh; SV Sawant & R Tuli. Chloroplast transformation for development of insect resistant plants. National Symposium on Plant Biotechnology: Towards Strategic Agriculture and Drug Development, CIMAP, Lucknow, India, March 15-17, 1999. Abs. No: P-5
- Singh, M & C Sharma. Molecular tools and the adulteration of herbal medicine: The road ahead. National Science Conference on Conservation of Bioresources and Their Sustainable Utilization, IHBT, Palampur, March 26-27, 1998, page 36-38.
- Singh, M. Construction of a chimeric plasmid vector for efficient integration, selection and expression of gusA reporter gene in tobacco chloroplasts. Indian Society of Agricultural Biochemists, Himachal Pradesh Krishi Viswavidyalaya, Palampur, India, October 24th, 1997. Abs. No: PS-48.
- Singh, M; R Woodgate & AS Levine. Plasmid encoded umuC mutants: molecular and genetic characterization. All India Cell Biology Conference & Symposia, 18th Annual Meeting, National Botanical Research Institute, Lucknow, India, February 13-15, 1995. Abs. on page-53.
- Gupta, SK; A Srivastava; M Singh; PK Singh; BK Sarangi & R Tuli. Regeneration of cotton through multiple shoot differentiation. All India Cell Biology Conference & Symposia, 18th Annual Meeting, National Botanical Research Institute, Lucknow, India, February 13-15, 1995. Abs. on page-128.
- Tuli, R; PVAL Ratnekar; J Kapila; A Srivastava; N Vijayalakshmi; HD Rananavare; HS Mishra; SK Gupta & M Singh. Development of transgenic plants for resistance to insect attack. International Symposium on Plant Molecular Biology & Biotechnology, International Center for Genetic Engineering & Biotechnology, New Delhi, India, December 14-17, 1994. Abs. on page-39.
- Singh, M; AS Levine & R Woodgate. Isolation and characterization of novel umuC mutants. NIH Research Day, National Institutes of Health, Bethesda, MD, USA, Sept. 23-24, 1992.
- McLenigan, MP; M Singh; S Hirschfield; AS Levine & M Protic. Differential expression of damage-specific DNA binding proteins in eukaryotic cells exposed to UV light. American Association for Cancer Research, Special conference on cellular responses to environmental DNA damage, Banff Spring Hotel, Banff, Alberta, Canada, December 1-6, 1991. Abs. No: A-71.
- Singh, M; AS Levine & M Protic. Expression of DNA repair associated proteins in carcinogen treated mammalian cells. NIH Research Day, National Institutes of Health, Bethesda, MD, USA, Sep. 23-24, 1991.
- Singh, M; SJ Pollins & JF John. Characterization of a tobramycin resistance gene in a Tn5 gene cluster. American Society for Microbiology, 91st Annual Meeting, Dallas, TX, USA, May 5-9, 1991. Abs. No. A-53.
- Singh, M; JNS Yadav & SC Sanyal. Isolation of a 60 mega-dalton plasmid of zoonotic importance from equine metritis. International Union of Microbiological Societies Congress: Bacteriology and Mycology, Osaka, Japan, September 16-22, 1990. Abs. No: PS76-05.
- Singh, M & JF John. A conjugative nosocomial R-plasmid expressing an isomeric ladder induced by tobramycin. American Society for Microbiology, 90th Annual Meeting, Anaheim, CA, USA, May 13-18, 1990. Abs. No: H-162.
- Singh, M & JF John. Inhibition of tobramycin induced supercoiling of a conjugative R-plasmid DNA from a nosocomial strain of Escherichia coli. Annual Student Research Day, Medical University of South Carolina, Charleston, SC, USA, Nov. 3, 1989. Abs. No: 20.
- Singh, M & JNS Yadav. Effect of various concentrations of acridinium ions on curing of R-plasmids. Indian Life Science Association, 8th National Symposium on Recent Advances in Life Sciences, Patna University Patna, Bihar, India, June 30-July 2, 1988.
- Singh, M; KK Singh; VK Saxena; M Ahmad & JNS Yadav. Emergence of resistance in Escherichia coli against some new antibiotics in the vicinity of Lucknow (India). International Congress of Microbiology, Annual Meeting, Manchester, England, September 3-7, 1986. Abs. No: PB7-12/56.
- Singh, M; M Ahmad & JNS Yadav. Behavior of drug-resistance markers among the strains of Escherichia coli in drug free media under laboratory conditions. Indian Science Congress Association, 73rd Annual Meeting, Delhi University, New Delhi, India, January 3-7, 1986, Abs. No: 62.
$papers won best poster award
#presentations by students