Teresa I. Shakespeare, Ph.D.

  • College: College of Arts and Sciences
  • Department: Biology Academic Department
  • Title: Associate Professor
  • shakespearet@fvsu.edu

TERESA I. SHAKESPEARE, PHD

Associate Professor
Fort Valley State University

Dr. Shakespeare is an associate professor in the Department of Biology at the Fort Valley State University, in Fort Valley, GA. She holds a Bachelor’s degree in Biological Sciences from Savannah State University and a doctorate degree in Physiology and Biophysics from Stony Brook University, where she studied gap junction communication in the lens, congenital cataractogenesis and lens growth. As a postdoctoral research fellow at St. Jude Children’s Research Hospital she studied the effect of Prox-1 on insulin producing beta cells, congenital abnormalities of pancreatic development and early onset Type 2 Diabetes. Dr. Shakespeare’s research background is firmly rooted in physiology, genetics, development, and cell biology.

She currently studies cellular dynamics and turnover of gap junction proteins in the cell. She is also interested in understanding the cellular and physical interactions of gap junction proteins in thrombosis and vascular occlusion event (VOE) in sickle cell patients.  Her research employs molecular and cellular techniques from the emerging fields of genomics and proteomics.  Dr. Shakespeare’s research has been sponsored by the American Society for Cell Biology, National Science Foundation, and the American Association for the Advancement of Science.

Dr. Shakespeare teaches Genetics, Histology, and Principles of Biology at Fort Valley State University. In addition to her teaching and scholarly interest, Dr. Shakespeare recently completed the Executive Leadership Fellowship at FVSU.  She dedicates much of her time and interest to the well-being of Fort Valley State University through her service, where she serves and chairs numerous institutional committees

In addition to her commitment to research, service and scholarly activities, Dr. Shakespeare enjoys educating and motivating her students, to first pursue undergraduate education in STEM disciplines, followed by advanced degrees in Biomedical Sciences.

Research Experience includes:

  • Gene Knockin and Gene Deletion
  • Genotyping
  • DNA Agarose Gel Electrophoresis
  • Protein SDS-Page Gel Electrophoresis
  • 2-D Gel Electrophoresis
  • Biophysical Electrophysiology
  • Histology and Immunohistochemistry
  • Polymerase Chain Reaction
  • Cell Culture
  • Cell Transfection
  • RNA Silencing
  • Flourescence Microscopy, Confocal Microscopy, TEM Microscopy
  • Immunoblotting

TERESA SHAKESPEARE, PHD.

Associate Professor of Biology

EDUCATION

B.S., Biology
Savannah State University, Savannah, Georgia (2001)

Ph. D., Physiology & Biophysics

Stony Brook University, Stony Brook, New York (2008)

Postdoctoral, Genetics

St. Jude Children’s Research Hospital, Memphis, TN (2009)

ACADEMIC POSITIONS

2014- Present

  • (Tenured) Associate Professor of Biology
    Fort Valley State University, Fort Valley, GA
  • American Society for Cell Biology
    Sponsored Visiting Professor, Cell Biology and Physiology, School of Medicine, University of Pittsburgh, Pittsburgh, PA. (Summer Semesters 2011, 2012, and 2013, 2015 and 2016)

2009-2014
Assistant Professor of Biology, Fort Valley State University, Fort Valley, GA

2008-2009
Postdoctoral Research Associate, St. Jude Children’s Research Hospital, Memphis, TN

2003-2007
Graduate Teaching Assistant, Stony Brook University, School of Medicine, Stony Brook, NY

2002-2008
Graduate Research Assistant, Stony Brook University, School of Medicine, Stony Brook, NY

COURSES TAUGHT

  • BIOL 1108-Principles of Biology II, FVSU (since Fall, 2009)
  • BIOL 4253-Genetics, FVSU (since Summer, 2009)
  • BIOL 1104- Introduction to Biology, FVSU (since Spring, 2010)
  • BIOL 3234-Embryology, FVSU (since Summer, 2012)
  • BIOL 4263-Immunology, FVSU (since Spring, 2009)
  • BIOL 3223-Microbiology, FVSU (Summer, 2010)
  • BIOL 3254- Histology, FVSU (since Fall 2013)
  • BIOL 3223- Cellular & Molecular Biology (since Spring 2017)
  • HBY695- Medical Physiology, Stony Brook University, School of Medicine (Spring 2003-2007)
  • Research Methods, Stony Brook University (Summer 2004-2007)

LABORATORY RESEARCH EXPERIENCE

University of Pittsburgh, Pittsburgh, PA
Visiting Professor- (Summer Semesters 2011- 2012, and 2013, 2015 and 2016)
Research Focus- Connexin43 Cell-Cell Communication and the Characterization of Endoexocytosis Associated Membrane Trafficking

  • Cell Culture of Mammalian Cells
  • Transfection
  • Immunocytochemistry
  • Transmission Electron Microscopy
  • Time Lapse Imaging
  • Confocal Microscopy
  • Quantum Dot Analysis
  • Western Blotting
  • siRNA

St. Jude Children’s Hospital, Memphis, TN
Postdoctoral Associate (2008-2009)
Research Focus- Characterizing the Role and Regulation of Prox-1 Homeobox Gene in the Developing Pancreas

  • Animal Husbandry and Genetic Breeding of Mice
  • Experienced in glucose and insulin tolerance testing in mice
  • Cell Culture of Mammalian Cells
  • Tissue Preparation for Histology
  • Immunohistochemistry (Fluorescence Microscopy)
  • Protocol development for Islet isolation
  • Northern and Southern Blotting

Stony Brook University, Stony Brook, NY
Doctoral research advised by Dr. Thomas W. White (2002-2008)
Connexin50 Intercellular Communication and Its Interaction with Mitogen Activated Protein Kinase Signaling: Implications for Postnatal Lens Growth

  • Mastered a wide array of molecular biology techniques including DNA manipulation, tissue extraction of genomic DNA, sub-cloning, PCR, in vitro preparation of RNA transcripts and analysis of protein expression by western blotting.
  • Proficient in the use of in vitro and in vivo animal preparations to study integrative organ systems physiology (mice, amphibians, and zebrafish), dual-cell voltage clamp electrophysiological procedures, cellular microinjections, micro-dissections and animal surgery
  • Proficient in Immunohistochemistry (IHC) technology, micro-dissection, sectioning and counterstaining of tissue, TUNEL Apoptosis Analysis, Caspase 3- detection, and 5-Bromo-2- deoxyuridine (BrdU) injection in mice
  • Experienced in laboratory safety and regulated waste management
  • Experience in Teaching/ Laboratory Scheduling and Management

Stony Brook University, Stony Brook, NY (2001)
Undergraduate Summer Intern research advised by Dr. M. Raafat El-Maghrabi
Expression of Skeletal Muscle 6-Phosphofructo-2-Kinase/Fructose-2, 6-Bisphosphatase in E.coli and Separation of the two Domains by Thrombin Cleavage

  • DNA manipulation
  • Tissue Extraction of Genomic DNA
  • Molecular sub-cloning
  • PCR
  • Nickel Column Protein Purification

Savannah State University, Savannah, GA (2000-2001)
Undergraduate research advised by Dr. Harpal Singh
Aniline Analog Induced Change in Canine Erythrocyte Membrane Proteins

  • Erythrocyte ghost cell preparation
  • SDS PAGE and western blotting
  • Cellular Histology

AWARDS/HONORS/FELLOWSHIPS/SCHOLARSHIPS

  • 2013- 2015: National Institutes of Health (NIH PRIDE Fellow)
  • 2011 & 2012: American Society for Cellular Biology Visiting Scholar/Professorship Award Fellow
  • 2012: American Society for Cellular Biology Linkage Fellow
  • 2009: St. Jude Postdoctoral Fellow Award
  • 2002- 2008: W. Burghardt Turner Pre- Doctoral Fellow
  • 2001: Summer Internship, Alliance for Graduate Education and the Professoriate Summer Research Institute, Stony Brook, NY.
  • 2000: Summer Internship, Minority Medical Education Program. University of Virginia, Charlottesville, VA

PROFESSIONAL ACTIVITIES

Departmental and University Committee Member:

  • Undergraduate Research Program Advisory Council: Committee Co- Chair (Fall 2016- present)
  • Campus Prioritization Academic Taskforce: Committee Chair (fall 2016-present)
  • Institutional Promotion and Tenure Committee: (Acad. Year 2015)
  • College of Arts and Sciences Promotion and Tenure Committee: (Acad. Year 2016)
  • of Biology Promotion and Tenure Committee: Chair (Acad. Year 2016)
  • of Biology Promotion and Tenure Committee: Chair (Acad. Year 2015)
  • Grant Review Panelist: NSF Panelist for Cellular and Molecular Biology (2014- present)
  • NIH Study Section for PRIDE (Programs for Research in Diversity Enhancement) (2012-2014)
  • Faculty Senate Member: Representative for Department of Biology, FVSU (2012-2016)
  • Master’s Thesis Committee Examiner: (Biotechnology) Whitley Stewart, Fort Valley State University (2011-2013)

Assessment’s Coordinator

  • Department of Biology, FVSU (Since Fall 2010)
  • University System of Georgia (USG) Core Curriculum Revision Committee (Nature Sciences and Mathematics Committee Chair-Area D), FVSU, (Since Fall 2010)
  • Committee Chair for Department of Biology Faculty Recruitment, FVSU, (December 2009)
  • FVSU QEP Curriculum Enhancement Committee for Critical Thinking in Biology, (Since Summer 2011)

Student Organization Advisor

  • Beta Kappa Chi (Co-advisor)
  • Medical Association for Minority Pre-Med Students
  • PSLSAMP (Faculty Mentor)
  • FVSU Pre-Medical Students Pipeline (Co-advisor)
  • National Institute of Science (Advisor)

Judging Science Presentations

  • Judged graduate and undergraduate oral presentations at PSLSAMP symposium (2009- present) Beta Kappa Chi and National Institute of Science Joint Annual Meeting (2009- present)
  • Annual Biomedical Research Conference for Minority Students (ABRCMS) (2010- present)
  • FASEB Experimental Biology 2004; Stony Brook Annual Graduate (AGEP) Symposium (2005-2008)

Professional Society Memberships

American Society for Cell Biology (ASCB), American Physiological Society (APS), Alliance for Graduate Education and the Professoriate Community of Science Mentors, National Postdoctoral Association, Beta Kappa Chi Honor Society (BKX), National Institute of Science (NIS), and American Association for University Professors (AAUP).

Service to National Society

American Society for Cell Biology (ASCB) Minority Affairs Committee Member (2014- present)

CONFERENCES ATTENDED BY MENTORED STUDENTS

  • Parker, Lauren (2016). Fort Valley State University Head Start Impact Study: Surveillance of Factors that Influence Health and Learning in Pre-School Children [abstract /poster presentation]. In Annual Biomedical Research Conference for Minority Students (ABRCMS); 2016 November 7-10; Tampa, FL. 2016.
  • Harper, Jordan (2012). Gap Junction Protein Phosphorylation and Annular gap Junction Formation [abstract/ oral presentation]. In Annual Biomedical Research Conference for Minority Students (ABRCMS); 2012 November 7-10; San Jose, CA: 2012. P.27 Abstract nr. O09.
  • I. Shakespeare, B. M. Nickel, S. J. O’Neil, J. Harper, S. A. Murray (2012). Analysis of Annular Gap Junction Vesicle Fission. Mol. Biol. Cell (suppl), Abstract No. 1309. Presented at the American Society for Cell Biology Annual Meeting. December 15-19, 2012, San Francisco, CA.
  • Harper, Jordan (2013). Gap Junction Protein Phosphorylation and Annular gap Junction Formation [abstract/poster presentation]. In: Fort Valley State University 3rd Annual Research Day “Scholarship in Action”; 2013, Apr 12; Fort Valley, GA. p 7. Abstract p23.

SPEAKER INVITATION

  • Stony Brook University, Center for Inclusive Education, State University of New York at Stony Brook, Stony Brook, NY. “Women’s History Month, Character, Courage, and Commitment Lecture Series. March 2014.
  • American Society for Cell Biology, San Francisco, CA. “Analysis of Annular Gap Junction Vesicle Fission.” 1st Annual Visiting Professor Lecture Series.  December 2012
  • American Society for Cell Biology (Minority Affairs Committee), Denver, CO. “Preparing for the Great Transition: From Graduate School to Postdoc and Beyond.” December 2011
  • FVSU Critical Thinking and Research Day Symposium, Fort Valley, GA. “The Fort Valley State University Head Start Impact Study: Pre-School Obesity Factors” April 2012
  • Jude Children’s Research Hospital National Symposium, Memphis, TN “Potential Interactions between Connexin50 Intercellular Communication and Mitogen Activated Protein Kinase Signaling in Lens Growth.” May 2008.

EXTRAMURAL FUNDING

Ongoing Research Support

  • Fort Valley State University- President’s Research Enhancement Grant (PREP) – Shakespeare (PI)- 01/06/2017
  • National Science Foundation (NSF) – Research Opportunity Award (ROA) – Murray and Shakespeare (PI)- 06/01/16

Submitted/Pending Research Support

National Institutes of Health (NIH) – Biobehavioral and Technological Interventions to Attenuate Cognitive Decline in Individuals with Cognitive Impairment or Dementia (R15) – Jefferson and Shakespeare- submitted 02/12/2017

Completed Research Support

  • American Society for Cell Biology /MAC- Visiting Professorship Award- Shakespeare (PI), 06/2011
  • American Society for Cell Biology /MAC- Visiting Professorship Award- Shakespeare (PI), 06/2012
  • American Society for Cell Biology /MAC- Minorities Affair Linkage Fellowship- Shakespeare (PI), 07/2012- 06/30/13
  • National Science Foundation (NSF) – Research Opportunity Award (ROA) – Murray and Shakespeare (PI), 05/01/14
  • National Science Foundation (NSF) – Research Opportunity Award (ROA) – Murray and Shakespeare (PI), 05/01/15
  • National Science Foundation (NSF)/ American Association for the Advancement of Science (AAAS) Women’s International Research Collaborations (WIRC) for Minority Serving Institutions (MSIs) – Shakespeare (PI), 12/2012- 12/2015
  • American Society for Microbiology (ASM) LINK Award- OPTIMIST: Developing an Online Portal for Student Mentoring, 01/2015
  • 2015: American Society for Microbiology (ASM) LINK San Antonio, TX. – Travel Award-
  • 2012: NSF- Women of Color in Stem, Purdue University- Travel Award
  • 2012: Annual Biomedical Research Conference for Minority Students, San Jose, CA. – Judges Subsidy Travel Award
  • 2012: American Society for Cell Biology, San Francisco, CA. – Travel Award
  • 2011: American Society for Cell Biology, Denver, CO.- Travel Award

 

PEER REVIEWED PUBLICATIONS

  • Teresa Shakespeare and Sandra A. Murray, Distribution of Clathrin and AP-2 in the Region of Gap Junction Plaques: Preferential Localization on the Inner Surface of One of the Contacting Cell Pairs. Journal of Cell Science (2017) ( publication submitted)
  • Sandra A. Murray and Teresa Shakespeare, Immunoflourescence: Applications for Analysis of Connexin Distribution and Trafficking. ©CRC Press, Boca Raton, Florida. (2016).
  • Beth Nickel, Marie Boller, Kimberly Schneider, Teresa Shakespeare, Vernon Gay and Sandra A. Murray (2013). Visualizing the effect of Dynamin Inhibition on Annular Gap Vesicle Formation and Fission. Journal of Cell Science 126, 2607–2616, 2013 doi: 10.1242/jcs.116269
  • Shakespeare TI, Sellitto C, Li L, Rubinos C, Gong X , Srinivas, White TW. Interactions between Connexin50 and mitogen activated protein kinase signaling in lens homeostasis. Mol Biol. Cell 2009 May; 20 (10): 2582-92.
  • Shakespeare, TI, Mathias RT, and White TW. Connexin Biology: The Role of Gap Junctions in Disease. Connexins: A Guide, © Humana Press, New York. May 2009.
  • Cheng S, Shakespeare T, Mui R, White TW and Valdimarsson G. (2004). Zebrafish Cx48.5 is required for Normal Lens Development. J. Biol Chem. 279, 36993-03.

ABSTRACTS FOR CONFERENCE PRESENTATIONS

  • Shakespeare TI, Sellitto C, Li L, Rubinos C, Gong X , Srinivas, White TW. “Potential Interactions between Connexin50 Intercellular Communication and Mitogen Activated Protein Kinase Signaling in Lens Growth.” Mol. Biol. Cell (suppl), Abstract No . American Society for Cell Biology Annual Meeting. December 2007, Washington, DC.
  • Beth Nickel, Marie Boller, Kimberly Schneider, Teresa Shakespeare, and Sandra A. Murray (2011). “Ultra structural and Live-Cell Analysis of Dynamin’s Role in Gap Junction Plaque Endoexocytosis.” Mol. Biol. Cell (suppl), Abstract No. 1317. . American Society for Cell Biology Annual Meeting. December 7-10 2011, Denver , CO.
  • Teresa I. Shakespeare, PhD, Beth M. Nickel, BS, Shakira J O’Neil, BS. The Life and Times of an Annular Gap Junction Vesicle: Formation And Fission [abstract]. In: Translation Science; 2012 Oct 3-5; Pittsburgh, PA: p 65. Abstract nr 65. Presented at the Translation Science 2012. October 3-5, 2012, Pittsburgh, PA.
  • Shakespeare, TI. (2012, December, 17). Visualizing the Effect of Dynamin Inhibition on Annular Gap Vesicle Formation and Fission. ASCB First Annual Visiting Professor Lecture Series, USA. America Society for Cell Biology Annual Meeting. December 15-19, 2012, San Francisco, CA. (Invited Speaker)
  • Teresa I. Shakespeare. Tracking Gap Junction Protein: Monitoring the Assembly, Trafficking and Turnover of Annular Gap Junction Vesicles [abstract]. In: Fort Valley State University 3rd Annual Research Day “Scholarship in Action”; 2013, Apr 12; Fort Valley, GA. p 38. Abstract nr O5. (Oral Presentation)
  • Teresa I. Shakespeare, Shakira J. O’Neil, and Sandra A. Murray. Death of the AJG: Morphological and Dynamic Changes [abstract]. In: Local Traffic 2013: 12th Annual Pittsburgh Symposium on Intracellular Membrane Traffic; 2013 May 5; Pittsburgh, PA: 2013. p 20.
  • Teresa I. Shakespeare, Beth Nickel, Shakira J. O’Neil, and Sandra A. Murray. Annular Gap Junction Vesicle Processing: Formation to Degradation [abstract]. In: International Gap Junction Conference: Connexins, Pannexins, and Innexins; 2013 July 13-18; Charleston, SC: 2013. p 148.
  • Teresa I. Shakespeare, Beth Nickel, Shakira J. O’Neil, and Sandra A. Murray (2013). The Fate of Annular Gap Junction Vesicles: Lysosomal Degradation Mol. Biol. Cell      (suppl), Abstract No. 1214. American Society for Cell Biology Annual Meeting. December 14-18, 2013, New Orleans, LO.
  • Steed*, M., Shakespeare**, T. I., Nickel,B., and Bisher, M., and Murray, S.A. Unraveling the Life and Times of Gap Junctions: Comparison of Gap Junction Structures with High Resolution, Transmission and Light Microscopy, Abstract No. 1222. American Society for Cell Biology Annual Meeting. December 6-10, 2014, Philadelphia, PA.
  • Analysis of Annular Gap Junction Vesicle Processing. Murray, Sandra1., Bell, Cheryl1., Steed, Mesia2., Shakespeare, Teresa3., Bisher, Margaret1.1Department of Cell Biology University of Pittsburgh, School of Medicine. 2Department of Biological Science Winston Salem University. 3Department of Biology Fort Valley State University: International Gap Junction Conference: Connexins, Pannexins, and Innexins; 2015 March 28- April 2; Valparaiso, Chile: 2015. p 74.
  • Bell, T. Shakespeare, M. Steed, M. Bisher, and S. Murray. The Role of Clathrin and Dynamin in Annular Gap Junction Vesicle Formation and Fission Processes. [abstract]. In: Local Traffic 2015: 14th Annual Pittsburgh Symposium on Intracellular Membrane Traffic; 2015 May 3; Pittsburgh, PA: 2015. p 32.