Nirmal Joshee, Ph.D.

PUBLICATIONS

Peer reviewed articles:

1. Khatri Poonam, and Nirmal Joshee. 2024. Somatic embryogenesis and shoot organogenesis in peanut cv. ‘Georgia-12Y’ and successful transfer to the soil. PLoS ONE 19(12): e0315060. https://doi.org/10.1371/journal.pone.0315060.
2. Nash, AOM, Joshee, N., Sherman, S., Lessl, J. T., & Coolong, T. 2024. Accumulation and histochemical localization of cadmium in hemp (Cannabis sativa L.) leaf and root tissue. HortScience, 59(8), 1150-1157. Retrieved Jul 31, 2024, from https://doi.org/10.21273/HORTSCI17930-24
3. Khatri P, Joshee N. 2024. Effect of picloram and desiccation on the somatic embryogenesis of Lycium barbarum L. Plants, 13, 151. https://doi.org/10.3390/ plants13020151
4. Manokari M, Jayaprakash K, Cokul Raj M, Abhijit Dey, Mohammad Faisal, Abdulrahman Abdullah Alatar, Joshee N, Mahipal Singh Shekhawat. 2023. In vitro micro-morphometric growth modulations induced by N6 cytokinins (Meta-Topolin and 6-benzylaminopurine) in Ceropegia juncea Roxb.: A rare medicinal climber.  South African J Botany 157:656-666.
5. Bharadwaj B, A. Mishegyan, S. Nagalingam, A. Guenther, N. Joshee, SH. Sherman*, C. Basu. 2023. Physiological and genetic responses of lentil (Lens culinaris) under flood stress. Plant Stress 7: 100130. https://doi.org/10.1016/j.stress.2023.100130
6. Manokari M., Cokul Raj M, Dey A., Mohammad Faisal, Alatar Abdulrahman A, Joshee N, Shekhawat Mahipal S. 2023. Improvements in morpho‑anatomical traits of adventitious roots of Hedyotis bifora (L.) Lam. using silicon nanoparticles. Silicon https://doi.org/10.1007/s12633-023-02484-6
7. Manokari M, Cokulraj M, Dey A, Faisal M, Alatar AA, Joshee N, Shekhawat MS (2023) The micro-morpho-anatomical changes in leaf structure of plantlets during in vitro propagation (micropropagation) of Gardenia jasminoides J. Ellis. Vegetos. https://doi.org/10.1007/s42535-023-00577-6
8. Jayaprakash K, Manokari M, Cokulraj M, Dey A, Faisal M, Alatar AA, Joshee N, Shekhawat MS. 2023.Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellataHaw. in response to meta-topolin Plant Cell Tissue and Organ Culture. https://doi.org/10.1007/s11240-023-02447-0.
9. Viswanathan P, Gosukonda, JS, Sherman SH, Joshee N, and Gosukonda rm. 2022. Prediction of in vitro organogenesis of Bacopa monnieri using artificial neural networks and regression models. Heliyon DOI: HYPERLINK "https://doi.org/10.1016/j.heliyon.2022.e11969" https://doi.org/10.1016/j.heliyon.2022.e11969
10. Sherman, SH and N. Joshee. 2022. Current status of research on medicinal plant Scutellaria lateriflora: A review. J Medicinally Active Plants. 11, (1):22-38. https://scholarworks.umass.edu/jmap/vol11/iss1/3
11. Bewg, W. Patrick, Scott A. Harding, Nancy L. Engle, BN Vaidya, Jingyin Yu, Ran Zhou, Jacob Reeves, Thomas W. Horn, Nirmal Joshee, Jerry W. Jenkins, Shengqiang Shu, Kerrie W. Barry, Yuko Yoshinaga, Jane Grimwood, Robert J. Schmitz, Jeremy Schmutz, Timothy J. Tschaplinski, and Chung-Jui Tsai. 2022. Multiplex knockout of trichome-regulating MYBs in poplar affects light sensitivity and triterpene accumulation. Plant Physiology, kiac128, HYPERLINK "https://doi.org/10.1093/plphys/kiac128" https://doi.org/10.1093/plphys/kiac128
12. Irvin L, Zavala Ortiz Y, Rivera Rivera K, Vaidya BN, Sherman Samantha H, A. Negrón Berríos J, Joshee N and Arun. A. 2021. Micropropagation of rare Scutellaria havanensis Jacq. and preliminary studies on antioxidant capacity and anti-cancer potential. Molecules, 26, 5813 (1-12). 
13. Perry Zachary D, Saminathan T, Arun A, Vaidya BN, Basu C, Reddy Umesh K. and Joshee N. 2021.Transcriptome analysis of cambium tissue of Paulownia collected during winter and spring. Diversity 13: 423, https://doi.org/10.3390/d13090423
14. Samantha H Sherman, Brajesh N Vaidya, and N. Joshee. 2021. Micromorphological studies in an anxiolytic medicinal plant, Scutellaria lateriflora L. J. Food and Agricultural Research 1(1):1
15. Wright, Robert E. III, Lubana Shahin, V Gogineni, Z Hussain, A Naeem, S Sadasivan, I Sinha, M Neely, Sharon K. Michellhaugh, S Mittal, N Joshee, and P Parajuli. 2021. Scutellaria extract inhibits proliferation and migration of brain-metastatic lung cancer cells via regulation of multiple signaling pathways. J. Medicinally Active Plants 10(1):32-41. 
16. Bajaj, R., Irvin, L., Vaidya, B.N., Dhekney, S. D., Joshee, N. 2021. Optimizing plant regeneration and genetic transformation of Paulownia elongata. Biocatalysis and Agricultural Biotechnology, 33:1-8, 
17. Zakaria, IA, Sherman, Samantha H, Vaidya Brajesh N, Joshee, N. 2021. In vitro propagation and synseed mediated short-term conservation of Scutellaria alpina L. and S. altissima L. Plant Cell Culture and Micropropagation, 16:e163.
18. Wang Yi, Nadeem F, Xu G, Zhang Q, Joshee N, Tahir N. 2020. Modifying crystallinity, and thermo-optical characteristics of Paulownia biomass through ultrafine grinding and evaluation of biohydrogen production potential. Journal of Cleaner Production, 269:1-9. https://doi.org/10.1016/j.jclepro.2020.122386
19. Wang Yi, N Joshee, W Cao, Q Wu, and Nadeem Tahir. 2019. Continuous hydrogen production by dark and photo co-fermentation using a tubular multi-cycle bioreactor with Paulownia biomass. Cellulose. 1-10. https://doi.org/10.1007/s10570-019-02468-z(0123456789().,)0123456789().,-
20. Shahin L, Phaal SS, Vaidya BN, Brown JE, N Joshee. 2019. Aronia (Chokeberry): an underutilized, highly nutraceutical plant. J Medicinally Active Plants 8(4):46-63.
21. Liu Bing, Yun-Hong Tan, Liu Su, Olmstead Richard, Min Dao-Zhang, Chen Zhi-Duan, Joshee Nirmal, Vaidya Brajesh, Chung Richard; Li Bo. 2019. Phylogenetic relationships of Cyrtandromoea and Wightia revisited: a new tribe in Phrymaceae and a new family in Lamiales. J. Systematics and Evolution, 58(1):1-17. https://doi.org/10.1111/jse.12513
22. Vaidya, B.N., B. Asanakunov, L. Shahin, H.L. Jernigan, N. Joshee and S.A. Dhekney. 2019. Improving micropropagation of Mentha x piperita L. using a liquid culture system. In Vitro Cell & Dev Biology – Plant. https://doi.org/10.1007/s11627-018-09952-4
23. Vaidya, B. N., Brearley, T. A., and Joshee, N. 2014. Antioxidant Capacity of Fresh and Dry Leaf Extracts of Sixteen Scutellaria Species. J. Medicinally Active Plants. (Accepted).
24. Yadav NK, Vaidya BN, Henderson K, Lee JF, Stewart WM, Dhekney SA, and Joshee N. 2013. A Review of Paulownia Biotechnology: A Short Rotation, Fast Growing Multipurpose Bioenergy Tree. American J Plant Science 4:2070-2082.
25. Clapa D, A. Fira, and N. Joshee. 2013. An efficient in vitro rooting and acclimatization method for horticultural plants using float hydroculture. HortScience 48(9): 1159-1167.
26. Tisserat B, L, Reifschneider, N, Joshee, and V. L. Finkenstadt. 2013. Evaluation of Paulownia elongata Wood Polyethylene Composites. Journal of Thermoplastic Composite Materials. 1-20 DOI: 10.1177/0892705713505856.
27. Tisserat B, L, Reifschneider, N, Joshee, and V. L. Finkenstadt. 2013. Properties of high density polyethylene – Paulownia wood flour composites via injection molding. BioResources 8(3):4440-4458.
28. Tisserat B, N. Joshee, A.K. Mahapatra, G.W. Selling, and V. L. Finkenstadt. 2013. Physical and mechanical properties of extruded poly(lactic acid)-based Paulownia elongata bio-composites. Industrial Crops and Products 44:88-96.
29. Patel P.S, Joshee. N, Rimando. A.M. and Parajuli, P. 2013. Anti-cancer scopes and associated mechanisms of Scutellaria extract and flavonoid wogonin. Current Cancer Therapy Reviews 9: 34-42.
30. Dandawate S, Williams L, Joshee N, Rimando AM, Mittal S, Thakur A, Lum L and Parajuli P. 2012. Scutellaria extract and wogonin inhibit tumor-mediated induction of Treg cells via inhibition of TGF-β1 activity. Cancer Immunol Immunother 61:701–711.
31. Parajuli P, N. Joshee, S. R. Chinni, A. M. Rimando, S. Mittal, S. Sethi and A. K. Yadav. 2011. Delayed growth of glioma by Scutellaria flavonoids involve inhibition of Akt, GSK-3 and NF-kB signaling. J Neurooncol. 101(1):15-24.
32. Tascan A, JW Adelberg, AM Rimando, M Tascan, N Joshee, AK Yadav. 2010. Hyperhydricity and flavonoid content of Scutellaria species in vitro on polyester-supported liquid culture systems. HortScience 45(10):1723-1728
33. Parajuli P, N. Joshee, A. Rimando, S. Mittal and A. K. Yadav. 2009. In vitro anti-tumor mechanisms of various Scutellaria extracts and constituent flavonoids. Planta Medica 75:41-48.
34. Joshee, N, SR Mentreddy, and AK Yadav. 2007. Mycorrhizal fungi and growth and development of micropropagated Scutellaria integrifolia plants. Industrial Crops and Production 25(2):169-177.
35. Joshee, N., B. K. Biswas, and A. K. Yadav. 2007. Somatic embryogenesis and plant development in Centella asiatica L., A highly prized medicinal plant of tropics. HortScience 42(3):633-637.
36. Joshee, N, Mutua, M, Zee, F., and Yadav, A. K. 2004. In vitro shoot bud induction and plantlet regeneration in guava as influenced by genotype. Acta Hort. (ISHS) 632:279-285.
37. Joshee N, Bastola D, and Cheng P-W. 2002. Transferrin-facilitated lipofection gene delivery strategy: characterization of the transfection complexes and intracellular trafficking. Hum. Gene Therapy 13:1991-2004.
38. Li, C, Joshee, N, Adler, K B and Cheng, P-W, 1999. Development of monoclonal antibodies against bovine mucin core-2β-6 N-acetylglucosaminyltransferase. Glycoconjugate Journal 16: 555-562.
39. Joshee N, Kisaka H, and Kitagawa Y, 1998. Isolation and characterization of a water- stress specific genomic gene, pwsi18, from rice. Plant Cell Physiol. 39 (1): 64 -72.
40. Takahashi R., Joshee N. and Kitagawa Y, 1994. Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress regulated genes in rice. Plant Molecular Biology. 26: 339-352.

Book chapters

1. Shekhawat MS, Joshee N. 2024. Silicon Nanoparticles (SiNPs) mediated morpho-histochemical improvements in the micropropagation of medicinal plants. In: Tissue Culture Techniques and Medicinal Plants: Enhancing Propagation and Production (Eds. Husen A, Pant M).  Taylor & Francis Group, LLC, 6000 Broken Sound Pkwy, NW. ISBN: 9781032555584, pp. 230-247
2. Sherman, Samantha H, Irvin L, Parajuli P, and Joshee N. 2023. Scutellaria species and cancer research: The way forward. Medicinal Plants for Cosmetics, Health and Disease (Eds. N. Lall,), pp. 199-213. Chapter 11. Taylor and Francis, CRC Press. DOI: 10.1201/9781003108375-11
3. Bajaj, R, Irvin, L, Vaidya, BN, Shahin, L, and Joshee, N. 2020. Optimization of micropropagation and genetic transformation protocols for Paulownia elongata: A short rotation fast growing bioenergy tree. Methods in Molecular Biology ‘Biofuels and Biodiesel’ (ed. C. Basu). Springer. Chapter 18, 271-284. DOI: https://doi.org/10.1007/978-1-0716-1323-8_18
4. Irvin L, Jackson C, Hill Aisha L, Bajaj R, Mahmoudi C, Vaidya Brajesh N, and Joshee N. 2019.Skullcaps (Scutellaria spp.): Ethnobotany and current research. Ch. 7. In: Medicinal Plants: From Farm to Pharmacy. N. Joshee, S. A. Dhekney, P. Parajuli (Editors). Springer. Pp. 141-168.
5. Wright Robert E III, Joshee N, and Parajuli P. 2019. Modulation of Tumor Immunity by Medicinal Plant or Functional Food-Derived Compounds. Ch. 12. In: Medicinal Plants: From Farm to Pharmacy. N. Joshee, S. A. Dhekney, P. Parajuli (Editors). Springer. Pp. 275-290.
6. Hussain F, Mittal S, Joshee N and Parajuli P. 2016. Application of Bioactive Compounds from Scutellaria in Neurologic Disorders. In: The Benefits of Natural Products for Neurodegenerative Diseases, Mohamed M, Mohamed A and Gullemin G (Eds), Springer-Verlag, Heidelberg Germany, Chapter 5.

Post-doctoral Scientist:

Poonam Khatri, PhD, 2022- From: Haryana, India BS in Life Science from Kurukshetra University, India MSc Biotechnology, Bangalore University, India PhD in Biotechnology from DCRUST, India Peanut Aspergillus interaction
Seethapathy G. Saroja, PhD, 2024- From: Tamil Nadu, India MSc., Biotechnology from St. Joseph’s College/Bharathidasan University. PhD in Pharmacognosy from University of Oslo, Norway Phytochemistry of Peanut phytoalexins/aflatoxins

Research Assistant:

Birat Sapkota, MS, 2022- From: Chitwan, Nepal BSc in Agriculture, Tribhuvan University, Kathmandu, Nepal Master of Science in Biotechnology Fort Valley State University, Fort Valley, GA, USA Morpho-histochemical studies of peanut cultivars

Current Graduate Students:

Akash Basnet, Fall 2024- From: Gulmi, Nepal BSc in Agriculture, Tribhuvan University, Kathmandu, Nepal Developmental biology of extra Floral Nectaries in Paulownia spp.
Pradeep Lakkarsu, Fall 2024- From: Telangana, India BSc, Professor Jayashankar Telangana State Agricultural University Hyderabad, India. Peanut Aflatoxin project.
Skyler Armstead, Spring 2025- From: Georgia, USA BS in Biology, Albany State University, Albany, Georgia. Reproductive biology of endangered Scutellaria montana